Solutions

Where possible, all liquid samples must be prefilled (in wellplates, capillaries etc). Additional solutions are treated as special cases and need to be discussed with the beamline team. 

Labeling

• Consider storage condition! If samples need to be stored wet, cooled, frozen, etc. a label that sticks and survives the storing process is needed!
• Use short and simple identifiers that match the provided spreadsheet. 
• Point out and label for which sample the solution is used. 
• If solution needs dilution (protein buffers etc), include dilution factor on label.

Proteins

• On buffer: please label for which samples it is (e.g. "Use for samples 1-23" ).
• Protein SEC: buffer max is 8 (in total, no combinations)!!! I.e.  This is because only up to 4 buffers can be automatically exchanged. Specifically this means after hours, all samples will be run in one of up to 4 buffers. When designing your experiment, please coordinate across sub-experiments to avoid having avoidable, non-essential minor variations in buffers. If you have a base buffer to be run as is, and with 3 different additives this counts as 4 buffers, because it is buffer exchanges with relate to automation.
• Min Volume for SEC buffer =  200 mL plus 3 x column volume per sample.  The per sample buffer consumption accounts for coflow requirement, and running a little more than single column volume for each sample to ensure full elution.
• If you supply concentrated buffer, please clearly label it with the required dilution! Ask your BL scientist about buffers on site (hazardous or production procedure), we might be able to provide common ones for you.
• Buffers are treated as sample for autoloader.
• Please communicate temperature expectations and camera length. 

Exceptions

In some cases, experiments where solutions need to be added just prior to running the samples can be accommodated. This is treated as an exception rather than the standard case! It will need more manual labour, which will cost time that will impact the amount of beamtime to run samples. We need clear and simple labeling to match samples and solutions (see guidelines above)! Use the comment box in the according google spreadsheet for detailed information! Discuss all exceptions with the beamline team prior to your beamtime. Contact information can be found here.