Ruby Fluorescence measurement

Laptop details

User: mx user

password: Beam109Line

Part 1: Laser and Detector Set up

1. Move the detector out of the way along the rail

2. Put the DAC into clamp and tighten the thumb screw

3. Plug spectrometer into laptop via USB

4. Slide the detector back along the rail so that it is sitting against the opening of the DAC

5. Turn the key on the laser PSS to Auto

6. Lock up the hutch

Part 2: Fluorescence Measurement Set up

Note: This will need to be done any time after the laptop has gone to sleep.

1. Open OceanView on the laptop

2. Select Spectroscopy Application Wizard

3. Select  Fluorescence

4. Click Next to select Active Acquisition

5. Change Scans to Average to 10 and click Next

6.  Click the big lightbulb icon at the top to store the dark spectrum

7. Tick the box for Add clean peaks to schematic

8. Click Finish

Part 3. Ruby Fluorescence measurement and analysis

1. Click the Play button. The graph should update every few second and just be showing noise.

2. Turn on the laser via the orange power box.

3. Click the up-down arrow icon to scale the graph height to fill the window. You should be seeing two peaks.  

4. Click the icon that looks like a table of data, and it will show your data in table format under the graph. From this you can find your max peak.

5. Click the Play/Pause button so that the collection stops after a single measurement. 

6. Right click on the graph and a blue line will appear with a readout of the wavelength under the graph. This is a good way to find your max peak, or easily see if the fluorescence peak has moved since your last data collection. You can also zoom in on a selected section of the graph by clicking the icon that looks like a magnifying glass with a 2 inside it. Note down the wavelength of the peak of the second doublet.

7. Open the Excel spreadsheet Ruby fluorescence measurement which is saved on the desktop. Make a folder under your name and save the spreadsheet under a new name for the relevant pressure and experiment. 

8. Enter the wavelength found in Step 6 into the bright green cell called Lambda R1. 

9. The pressure in the cell is given in column C. Save the spreadsheet again and close.