A number of objectives with various magnifications and numerical apertures (NA) are available for Users. Standard Set-Up:
Available Alternatives:
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We have a number of liquid cells available. Please contact a Beamline Scientist if you are considering a liquid cell for your experiment. NOTE: The PC2 laboratory is available for loading of the liquid cells as required for certain biological samples. Liquid Flow-Through Cell A Bioptechs flow cell designed for light microscopy has been adapted for FTIR microspectroscopy. The cell uses 40 mm windows, with a 2 mm thick drilled bottom window and a 1 mm thick standard top window. We currently have CaF2 available with ZnS windows ordered and awaiting delivery. The design uses a peristaltic pump to flow a film of media around live biological cells. Their response to changes in this media can then be measured in almost real time. Please contact a Beamline Scientist if you wish to use this sample holder. For more details on the cell itself, please see the Bioptechs website. Liquid Flow-Through Cell A Bioptechs flow cell designed for light microscopy has been adapted for FTIR microspectroscopy. The cell uses 40 mm windows, with a 2 mm thick drilled bottom window and a 1 mm thick standard top window. We currently have CaF2 available with ZnS windows ordered and awaiting delivery. The design uses a peristaltic pump to flow a film of media around live biological cells. Their response to changes in this media can then be measured in almost real time. Please contact a Beamline Scientist if you wish to use this sample holder. For more details on the cell itself, please see the Bioptechs website. Figure: Bioptechs liquid flow-through cell
Figure: [Left] Compression cell (open) with 13 mm CaF2 window. [Right] Fabricated CaF2 spacer (13 mm) with S-channels
Figure: Harrick cell shown in assembly order from left to right. The pathlength spacers are cut into semi-circles to provide the liquid flow channel, allowing for standard IR windows to be used in the setup.
Figure: [Left] Disassembled ATR flow-through cell with 5 mm ROC Ge crystal shown. [Centre] Ge ATR crystal in position in the flow-through cell (without the lid). [Right] ATR flow-through in position in the microscope using the Bruker 20× objective. |
Please contact a Beamline Scientist if you wish to use any of the listed accessories
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Our offline spectrometer is a Bruker Vertex70 coupled with an Hyperion3000 microscope that can be used for large low-resolution overview maps of your sample to quickly determine regions of interest before high-resolution measurements are acquired with the synchrotron light. Specifications:
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The online instrument uses OPUS 8.0 (Bruker) for measurement acquisition as well as some in-house written graphical user interfaces (GUIs) for controlling the non-Bruker upgrades to the Hyperion microscope (e.g. motorised pinholes) and mirror movements for alignment of the beam / optimisation of signal. Note: Bruker spectrum files can be batch converted into the following file formats:
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We have a few commercial software packages available at the beamline for data processing
We are also working to develop our skills with the free software Orange, developed at the University of Ljubljana, Slovenia. [Demsar J et al. (2013) Orange: Data Mining Toolbox in Python, Journal of Machine Learning Research 14(Aug): 2349−2353] |