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← Back to Mail in Samples & Templates

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  • preferably supply all samples in dispensed into well plates all prefilled, frozen where possible.
  • special cases discussed with BL scientist in charge
  • If we need to do manual labour it'll cost time (from beamtime) and labour. 
  • counted as exceptions
  • labelling guidelines! simple labels (simple numbers (1,2,3,...) or simple alpha-numeric codes (A1, B3, E22), only .
    • You might be used to your own systems of detailed, descriptive labels that are intuitive to you.
    • However Eppendorfs containing complex scientific details can be difficult for us to read, especially when frozen, often contain only subtle variations between samples and are likely to be confusing for other people to read who are not doing your science.
    • What we need are short, simple, easy to read identifier numbers or codes that matches your spreadsheet. Use decriptors in the spreadsheet if they help you, but the the filenname code is what will be used for runnign the samples.
    • If you use tubes (rather than pre-loaded plates), it will help us if you use ones designed for labeling rather than just plain clear plastic ones.
  • Consider sample condition! (frozen / wet needs labels that stick and survive!) 
  • On buffer: please label for which samples it is (e.g. "Use for samples 1-23" ).
  • Protein SEC: buffer max is 8 (in total, no combinations)!!! I.e.  This is because only up to 4 buffers can be automatically exchanged. Specifically this means after hours, all samples will be run in one of up to 4 buffers. When designing your experiment, please coordinate across sub-experiments to avoid having avoidable, non-essential minor variations in buffers. If you have a base buffer to be run as is, and with 3 different additives this counts as 4 buffers, because it is buffer exchanges with relate to automation.
  • Min Volume for SEC buffer =  200 mL plus 3 x column volume per sample.  The per sample buffer consumption accounts for coflow requirement, and running a little more than single column volume for each sample to ensure full elution.
  • If you supply concentrated buffer, please clearly label it with the required dilution! Conversation with BL scientist about buffers on site (hazardous or production procedure). Buffer treated as sample for autoloader. Temp expectations and camera length. 
  • Comment box in spreadsheet!
  • No labels