Solutionspreferably supply all samples in dispensed into well plates all prefilled, frozen where possible.special cases discussed with BL scientist in chargeIf we need to do manual labour it'll cost time (from beamtime) and labour. counted as exceptionslabelling guidelines! simple labels (simple numbers (1,2,3,...) or simple alpha-numeric codes (A1, B3, E22), only .- You might be used to your own systems of detailed, descriptive labels that are intuitive to you.
- However Eppendorfs containing complex scientific details can be difficult for us to read, especially when frozen, often contain only subtle variations between samples and are likely to be confusing for other people to read who are not doing your science.
- What we need are short, simple, easy to read identifier numbers or codes that matches your spreadsheet. Use decriptors in the spreadsheet if they help you, but the the filenname code is what will be used for runnign the samples.
- If you use tubes (rather than pre-loaded plates), it will help us if you use ones designed for labeling rather than just plain clear plastic ones.
Consider sample condition! (frozen / wet needs labels that stick and survive!)  Image Added
Where possible, all liquid samples must be prefilled (in wellplates, capillaries etc). Additional solutions are treated as special cases and need to be discussed with the beamline team. Labeling- Consider storage condition! If samples need to be stored wet, cooled, frozen, etc. a label that sticks and survives the storing process is needed!
- Use short and simple identifiers that match the provided spreadsheet.
- Point out and label for which sample the solution is used.
- If solution needs dilution (protein buffers etc), include dilution factor on label.
Proteins- On buffer: please label for which samples it is (e.g. "Use for samples 1-23" ).
- Protein SEC: buffer max is 8 (in total, no combinations)!!! I.e. This is because only up to 4 buffers can be automatically exchanged. Specifically this means after hours, all samples will be run in one of up to 4 buffers. When designing your experiment, please coordinate across sub-experiments to avoid having avoidable, non-essential minor variations in buffers. If you have a base buffer to be run as is, and with 3 different additives this counts as 4 buffers, because it is buffer exchanges with relate to automation.
- Min Volume for SEC buffer = 200 mL plus 3 x column volume per sample. The per sample buffer consumption accounts for coflow requirement, and running a little more than single column volume for each sample to ensure full elution.
- If you supply concentrated buffer, please clearly label it with the required dilution! Conversation with Ask your BL scientist about buffers on site (hazardous or production procedure). Buffer , we might be able to provide common ones for you.
- Buffers are treated as sample for autoloader. Temp
- Please communicate temperature expectations and camera length. Comment box in spreadsheet!
ExceptionsIn some cases, experiments where solutions need to be added just prior to running the samples can be accommodated. This is treated as an exception rather than the standard case! It will need more manual labour, which will cost time that will impact the amount of beamtime to run samples. We need clear and simple labeling to match samples and solutions (see guidelines above)! Use the comment box in the according google spreadsheet for detailed information! Discuss all exceptions with the beamline team prior to your beamtime. Contact information can be found here. |
