Our apology for previous MCT users that were used 5X and 20X objective lens. We did not take into account the tube lens magnification of 0.9X for 5X and 20X objective lenses of Whitebeam detector. Here is the correct information:
Obj. lens magnification | Tube lens magnification | Total magnification | FOV (mm2) | Effective pixel size (µm) |
|---|---|---|---|---|
1X | 1X | 1X | 16.6 x 6.6* | 6.5 |
5X | 0.9X | 4.5X | 3.7 x 3.1 | 1.444 |
20X | 0.9X | 18X | 0.92 x 0.78 | 0.361 |
The incorporated meta data that were collected then need to be corrected, because the effective pixel size was not correct. The correct information of the meta data is important if you want to do quantitative analysis or stitching data correctly. Here is the procedure to change the information in the meta data.