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IR analysis can be run using transflection, which requires sections to be laid onto IR reflective slides e.g. Kevley MirrIR slides or ITO coated glass, instead of straight transmission. MirrIR slides are much cheaper than IR transmissive slides such as calcium fluoride and have the larger dimensions of normal microscope slides (1 x 3 inches), so you can potentially lay 1-3 sections per slide. There are inherent problems with this method however, in that your sample e.g. if working with tissue sections, have to be extremely uniform and ideally 4 µm thick, rather than the 5-8 µm thick required for transmission with a bit more leeway in the uniformity. Results are also more difficult to interpret using this method. We recommend the following papers discussing the potential issues with transflection analysis for further information. P. Bassan, J. Lee, A. Sachdeva, J. Pissardini, K.M. Dorling, J.S. Fletcher, A. Henderson, P. Gardner. Analyst, 138, 144 (2013) K. Malek, B.R. Wood, K.R. Bambery. FTIR Imaging of Tissues: Techniques and Methods of Analysis; Chapter 15. in book M. Baranska (ed.), Optical spectroscopy and computational methods in biology and medicine. Springer Science, 2014. M. Miljkovic, B. Bird, M. Diem. Analyst, 137, 3954 (2012) |
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