...
| Expand | ||
|---|---|---|
| ||
Our FTIR microscope typically operates in the mid-IR region from 750 cm-1 up to 3800 cm-1. The long wavelength range can also be extended to 550 cm-1 if required using the wide band wideband detector but with a 10× loss in sensitivity, and a larger aperture size is required, compromising spatial resolution. We recommend that this detector only be used if you are interested in specific bands within the 900-550 cm-1 range. Example figures coming soon!spectra acquired with the narrowband and wideband detector on the same sample can be found HERE. |
| Expand | ||
|---|---|---|
| ||
When preparing your beamtime proposal, it is important that you demonstrate that there is a genuine need for the high lateral spatial resolution achievable at the beamline. The beam can be focused to a spot size ranging from ~1-20 µm, depending on the experimental configuration and is ideal for raster mapping complex heterogeneous polymeric samples, single cell, or complex biological tissue, for example. Ideally, you will specifically state the spatial resolution that you required, in microns. Remember that as the wavelength of mid-IR radiation is in the 3-10 µm range, this will thereby essentially limit the smallest sized objects that can be individually resolved using this beamline. The size of the beam, according to the sampling mode as well as the objective and pinhole used, can be found HERE . Experiments requiring a spot size of 20 µm or greater, or that require 'bulk' or average measurements per sample, will have no advantage using the synchrotron and can be carried out on a lab-based IR microscope. However, If you can show that desired results were unsuccessful using a lab-based IR microscopy, this can be advantageous for your application to use our beamline. Furthermore, we now offer time resolved IRM, or rapid scan analysis, with the ability to measure up to 65 spectra/sec at a resolution of 16 cm-1, and a scanner velocity of 160 kHz. Requirements of the time resolved spectroscopy could justify the requirement of the synchrotron IRM beamline without the need for spatial resolution. Please contact an IRM team member if you are unsure whether your experiment would benefit from use of the synchrotron. |
...