Tissue/Cell Culture Lab
A lab dedicated to mammalian tissue culture work (no bacteria/fungi). Features class II BSC’s, CO2 incubators, centrifuges, microscope and autoclave. Liquid nitrogen dewars* are available in this lab.
*Use must be discussed in advance of EA submission with beamline scientists and lab staff.
Requires booking in advance (>4 weeks).
Note: This lab is a biological lab, use hooks provided for re-use of gowns, no touching handles or taps with gloves, do not put anything porous (e.g. cardboard, Styrofoam) on the ground, wash hands before exit.
Use of this lab in advance of or post-beamtime site allowance (more than 2 days) will require an extended access lab EA - see info here: *****
Tissue Culture Lab entry requirements:
Valid online lab induction PLUS
Valid onsite chemistry lab induction PLUS
Valid onsite tissue culture lab induction
Items/Equipment in lab | Details | Notes |
|---|---|---|
2x Biosafety Cabinet | For any work that requires Class II containment. UV cycle must be run with sash closed when session complete | Follow SOP available Serviced annually |
4x CO2 Incubators | 2x heat sterilization; 2x hydrogen peroxide sterilization Must have at least 4 days between bookings to clean and run sterilization program | Door sheets must be filled out when putting cell lines in or removing them. |
Autoclave |
| Additional training required. Follow SOP available Serviced annually |
Water bath | Temp range **** | Includes bottle weight |
Microscope |
| Slides and slide cover available, some well plates may be available - see Lab Consumables page ***** |
Centrifuge **** | Different rotors available | 50ml, 15ml falcon tubes, Eppendorf |
Mini spin centrifuge | For Eppendorf tubes only | 2.5ml |
Racks | Different sizes available |
|
Cell counting kits & double containment | Counter, slide covers, ****, trypan blue stain | Plastic container (clipped shut) for double containment when transporting cells outside lab |
RO/Ultra-pure water; Fridge/Freezer, ULT freezer (-80) | Located in the lab for easy access. Closest ULT freezer is in the chemistry lab | RO water unit only dispenses up to 5L. Additional RO water can be dispensed from the chemistry lab |
Spray bottles/squeeze bottles for water, 80% ethanol or cleaning | 500mL | Discuss options with lab staff |
Scales | ThermoScientific | Can be placed in room |
PPE | *Many options available - see PPE page ***** | Discuss options with lab staff if required |
Consumables/Stationery | *Many options available - see lab consumables page ***** | Discuss options with lab staff if required |
Cell Waste treated with antibiotics
Any liquid waste containing antibiotics should be decanted into the designated waste bottles for autoclaving (denaturing) and then once cooled, flushed down the sink with water.
Waste for autoclave cannot contain bleach or large amounts of alcohol-based solvents. Ensure waste label is filled out with contents.
Any liquid waste that has not been treated with antibiotics can have bleach added to denature and once >15mins has elapsed, flushed down the sink with water.
Ask lab staff for assistance if unsure.
Samples/packaging/chemicals brought onsite must be removed from this lab and returned to your home institute following the conclusion of lab use.
Minor waste (e.g. paper towel) can be disposed of in the clinical waste bin near the door, serological pipette tips must go in designated bins provided. All sharp items must go in a sharps container.
Some dedicated liquid waste containers can be provided (e.g. formalin; crystal violet etc.) but must be discussed prior to EA approval.
Ask lab staff for assistance if unsure.
Autoclaving is available if sterilization (e.g. tips) is required - include in your EA, additional training is required.