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Part 2: Fluorescence Measurement Set up
Note: This should only will need to be done once at the start of the day, unless you want to change the integration time or number of scans to averageany time after the laptop has gone to sleep.
Open OceanView on the laptop
Select Spectroscopy Application Wizard
Select Fluorescence
Click Next to select Active Acquisition
Change Scans to Average to 10 and click Next
Click the big lightbulb icon at the top to store the dark spectrum
Tick the box for Add clean peaks to schematic
Click Finish
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Click the Play button. The graph should update every few second and just be showing noise.
Turn on the laser via the orange power box.
Click the up-down arrow icon to scale the graph height to fill the window. You should be seeing two peaks.
Click the icon that looks like a table of data, and it will show your data in table format under the graph. From this you can find your max peak.
Click the Play/Pause button so that the collection stops after a single measurement.
Right click on the graph and a blue line will appear with a readout of the wavelength under the graph. This is a good way to find your max peak, or easily see if the fluorescence peak has moved since your last data collection. You can also zoom in on a selected section of the graph by clicking the icon that looks like a magnifying glass with a 2 inside it. Note down the wavelength of the peak of the second doublet.
Open the Excel spreadsheet Ruby fluorescence measurement which is saved on the desktop. Save it Make a folder under your name and save the spreadsheet under a new name for the relevant pressure and experiment.
Enter the wavelength found in step Step 6 into the bright green cell called Lambda R1.
The pressure in the cell is given in column C. Save the spreadsheet again and close.
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