Almost all protein crystallography groups have transitioned to robot mounting. The following instructions will demonstrate the complete workflow before, during, and after, your experiment for the average user.
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It’s important to have a list of which pucks (ASP####) your samples are associated with. These pucks are loaded into the sample dewar that contain 3 adaptors (Left, Middle, Right). Pucks list can be access via Quick Access in the Toolbar (bottom left), and select ‘Robot Dewar Content’ in the Quick links prompt. The pucks can also be viewed from your Beamline Ready email by click on the hyperlink ‘Pucks List’.Note down which pucks numbers belong to you and the position that they are held in the sample dewar. For e.g. ASP0559 is in ‘Left’ Position D and ASP0569 is in 'Middle' Position A.
If you don’t see your pucks and your CAP has sent more than 12 pucks to the beamline, it’s possible that you will need to request an adaptor change from the AS operators. Their contact details are in the Beamline Ready email, or on a laminated poster stuck on the wall.
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Collecting Data
This is the general workflow in collecting data on your protein crystals. You will need to launch 3 GUIs and a webpage. In the Toolbar, you will find the icons for: ‘MX Shutter Flux’, ‘MX Robot’, ‘MX Collect’.
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The Processing webpage can be opened from the Quick Access button in the toolbar, select ‘All processing results (dataset and indexing)’. It can also be reached by typing ‘processing/processing’ into a web browser.
1. Take over access of the MX robot
It’s a good time to let your CAP know that you are taking over data collection. The MX Robot interface is designed so that only one location (either on site or remote access) can control the robot at any given time.
In the MX Robot GUI (webpage), on the top right corner. If access is currently set to inactive, take over robot access by clicking on thebutton to change it to active.
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