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Phase-retrieved tomography result for an insect inside small Eppendorf tube (ID ~ 5mm). (a). 3D rendering of the reconstruction data, (b). 3D colour segmentation using Dragonfly software. The data was collected using 12.7 keV monochromatic (Ru/C stripes), in vacuo filtration – 0.2 mm diamond, with 1X objective lens in the imaging detector, 1.5 m sample to the detector distance, 1801 images collected over 180 deg in 0.1 deg steps, 30 flat fields & 30 dark currents, “step-&-shoot” mode – exposure time per frame 40 msec – total collection time ~100 min – total data size ~19 GB – file format TIFF. TIE-Home phase retrieval was applied on the projection images before 3D reconstruction.
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Carbon fibre sample (PETG - Glycol modified Polyethelene Terephthalate) was collected using monochromatic 20 keV, exposure time 100 ms, 361 projection images at 160mm 160 mm sample to detector distance. The tomography reconstruction was done using MCT recontruction pipeline, data presentation using ORS-Dragonfly software.